ProSci

B2M Antibody [SPM617]

Product Code:
 
PSI-33-457
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM617
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Non-Human Primates
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with B2M antibody (SPM617)
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Formalin-fixed, paraffin-embedded human cervical carcinoma stained with B2M antibody (SPM617)

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PSI-33-457-100ug100ug£765.00
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Further Information

Additional Names:
B2M Antibody: Beta-2-microglobulin
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the B2M antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Serum Beta-2 Microglobulin (B2M) levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Full length recombinant human protein was used as the immunogen for the B2M antibody.
NCBI Gene ID #:
567
NCBI Official Name:
beta-2-microglobulin
NCBI Official Symbol:
B2M
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P61769
User NOte:
Optimal dilutions for each application to be determined by the researcher