TGF alpha Antibody [TFGFa-1]

TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the TGF alpha antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. The epitope is known to be in the C-termial region of the protein.

Transforming growth factor alpha is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGFa is a mitogenic polypeptide. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGFa is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. [Wiki]

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Recombinant human full-length TGF protein was used as the immunogen for the TGF alpha antibody. (3)

7039

transforming growth factor, alpha

TGFA

Homo sapiens

Liquid

Protein G affinity chromatography

Growth Factor,Antibody Pairs, Functional Assays

P01135

Optimal dilutions for each application to be determined by the researcher