South Bay Bio

20S Constitutive Proteasome Assay Kit

Product Code:
 
SBB-KP0038
Supplier:
 
South Bay Bio
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Compound Screening
Shipping:
 
DRY ICE
Storage:
 
-20°C
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20S Immunoproteasome Activity Raw Data Output: Several wells of Immunoproteasome shown digesting LLVY, PAL, and ANW-AMC over time +/- 1x (40?M) inhibitor (ONX-0914).
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20S Immunoproteasome Activity Raw Data Output: Several wells of Immunoproteasome shown digesting LLVY, PAL, and ANW-AMC over time +/- 1x (40?M) inhibitor (ONX-0914).

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SBB-KP0038-KI011 Kit£586.00
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This product comes from: Switzerland.
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Further Information

Detection Type:
Fluorescent
EClass:
32160000
Handling Advice:
Aliquot to avoid freeze/thaw cycles.Avoid freeze/thaw cycles.
Kit Contains:
10x 20S Proteasome | 50x LLVY-AMC, Chemotrypsin-like activity | 50x WLA-AMC, beta5/PSMB5 specific substrate | 50x LLE-AMC, beta1/PSMB6 specific substrate | 50x Inhibitor (MG-132, 2mM) in 100% DMSO | 10x Reaction Buffer | 50x SDS(1.75%) in H20 | 100x free AMC Standard (40µM)
Long Description:
South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits beta1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of beta5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.
Package Type:
Vial
Product Description:
South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits beta1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of beta5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.
Specificity:
Readout: Endpoint/Kinetic Label or Dye: 7-Amino-4-methylcoumarin Detection Method: Fluorescence Substrate Properties: Protein-based Substrate. Excitation/Emission (nm): 345/445
Transportation:
Non-hazardous
UNSPSC Category:
Chemistry test kits or supplies
UNSPSC Number:
41116104