NSJ Bioreagents

Firefly Luciferase Antibody

Product Code:
 
NSJ-RQ5248
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DAB-6
Regulatory Status:
 
RUO
Application:
 
Western Blot (WB)
Storage:
 
Store the Firefly Luciferase antibody at -20°C.
1 / 2
Western blot testing of 1) 293T cells transfected with firefly luciferase, 2) human HeLa, 3) mouse NIH3T3 and 4) rat C6 lysate with Firefly Luciferase antibody. Predicted molecular weight ~60 kDa.
2 / 2
Immunofluorescent staining of transfected Firefly Luciferase-293 cells with Firefly Luciferase antibody (green) and DAPI (blue).
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Western blot testing of 1) 293T cells transfected with firefly luciferase, 2) human HeLa, 3) mouse NIH3T3 and 4) rat C6 lysate with Firefly Luciferase antibody. Predicted molecular weight ~60 kDa.
Immunofluorescent staining of transfected Firefly Luciferase-293 cells with Firefly Luciferase antibody (green) and DAPI (blue).

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NSJ-RQ5248-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:5000,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the Firefly Luciferase antibody should be determined by the researcher.
Description:
Firefly luciferase is the light-emitting enzyme responsible for the bioluminescence of fireflies and click beetles. The enzyme catalyses the oxidation of firefly luciferin, requiring oxygen and ATP. Because of the requirement of ATP, firefly luciferases have been used extensively in biotechnology. [Wiki]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to Firefly Luciferase was used as the immunogen for the Firefly Luciferase antibody.
Limitation:
This Firefly Luciferase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Firefly luciferase
Uniprot #:
P08659