ProSci

IGF1 Antibody

Product Code:
 
PSI-14-216
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using IGF1 antibody (14-216) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using IGF1 antibody (14-216) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IGF1 antibody (14-216) at dilution of 1:100 (40x lens).

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PSI-14-216-50uL50uL£433.00
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Further Information

Additional Names:
IGF1, OTTHUMP00000195084, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor 1, IGFI, IGF1A, IBP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IGF1 (NP_000609.1).
NCBI Gene ID #:
3479
NCBI Official Name:
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol:
IGF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P05019
User NOte:
Optimal dilutions for each application to be determined by the researcher.