ProSci

ACACA Antibody

Product Code:
 
PSI-16-133
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using ACACA antibody (16-133) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunofluorescence analysis of 293T cells using ACACA antibody (16-133) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of C6 cells using ACACA antibody (16-133) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of HeLa cells using ACACA antibody (16-133) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using ACACA antibody (16-133) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of 293T cells using ACACA antibody (16-133) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ACACA antibody (16-133) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using ACACA antibody (16-133) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37°C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining.

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PSI-16-133-50uL50uL£433.00
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Further Information

Additional Names:
Acetyl-CoA carboxylase 1, ACC1, ACC-alpha, Biotin carboxylase, ACACA, ACAC, ACC1, ACCA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human ACACA
NCBI Gene ID #:
31
NCBI Official Name:
Acetyl-CoA carboxylase 1
NCBI Official Symbol:
ACACA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 240kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13085
User NOte:
Optimal dilutions for each application to be determined by the researcher.