ProSci

ADRBK2 Antibody

Product Code:
 
PSI-18-466
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using ADRBK2 antibody (18-466) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.
2 / 3
Immunohistochemistry of paraffin-embedded rat spinal cord using ADRBK2 Antibody (18-466) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse brain using ADRBK2 Antibody (18-466) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using ADRBK2 antibody (18-466) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.
Immunohistochemistry of paraffin-embedded rat spinal cord using ADRBK2 Antibody (18-466) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ADRBK2 Antibody (18-466) at dilution of 1:100 (40x lens).

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PSI-18-466-50uL50uL£433.00
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Further Information

Additional Names:
Beta-adrenergic receptor kinase 2, Beta-ARK-2, G-protein-coupled receptor kinase 3, ADRBK2, BARK2, GRK3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ADRBK2 (NP_005151.2).
NCBI Gene ID #:
157
NCBI Official Name:
Beta-adrenergic receptor kinase 2
NCBI Official Symbol:
ADRBK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P35626
User NOte:
Optimal dilutions for each application to be determined by the researcher.