ProSci

IKK alpha Antibody

Product Code:
 
PSI-18-473
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using IKK alpha antibody (18-473) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of A549 cells using IKK alpha antibody (18-473). Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug IKK alpha antibody (18-473). Western blot was performed from the immunoprecipitate using IKK alpha antibody (18-473) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using IKK alpha antibody (18-473) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using IKK alpha antibody (18-473). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug IKK alpha antibody (18-473). Western blot was performed from the immunoprecipitate using IKK alpha antibody (18-473) at a dilition of 1:1000.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-18-473-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKK alpha (NP_001269.3).
NCBI Gene ID #:
1147
NCBI Official Name:
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol:
CHUK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 94kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
O15111
User NOte:
Optimal dilutions for each application to be determined by the researcher.