ProSci

DLG1 Antibody

Product Code:
 
PSI-23-649
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using DLG1 antibody (23-649) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using DLG1 antibody (23-649) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).

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PSI-23-649-50uL50uL£433.00
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Further Information

Additional Names:
DLG1, discs, large homolog 1 (Drosophila), DKFZp761P0818, DKFZp781B0426, DLGH1, SAP97, dJ1061C18.1.1, hdlg, discs large homolog 1, large homolog 1, presynaptic protein SAP97, synapse-associated protein 97, SAP-97
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).
NCBI Gene ID #:
1739
NCBI Official Name:
discs, large homolog 1 (Drosophila)
NCBI Official Symbol:
DLG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 114kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Neuroscience
Swissprot #:
Q12959
User NOte:
Optimal dilutions for each application to be determined by the researcher.