ProSci

PLK4 Antibody

Product Code:
 
PSI-23-947
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using PLK4 antibody (23-947) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (23-947) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PLK4 antibody (23-947) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (23-947) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (23-947) at dilution of 1:100 (40x lens).

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PSI-23-947-50uL50uL£433.00
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Further Information

Additional Names:
PLK4, polo-like kinase 4 (Drosophila), STK18, serine/threonine kinase 18, SAK, Sak, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).
NCBI Gene ID #:
10733
NCBI Official Name:
polo-like kinase 4
NCBI Official Symbol:
PLK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O00444
User NOte:
Optimal dilutions for each application to be determined by the researcher.