Bromocresol Green

3',3",5',5"-Tetrabromo-m-cresolsulfonephthalein; NSC 7817; BCG

Beige to yellow-brown powder.

76-60-8

32160000

solid

Protect from light and moisture.

H302-H312-H315-H319-H332-H335

InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3

FRPHFZCDPYBUAU-UHFFFAOYSA-N

Chemical. CAS: 76-60-8. Formula: C21H14Br4O5S. MW: 698.01. Bromocresol green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualization of the compounds with functional groups whose pKa is below 5.0. One major use is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue). Visual transition interval: pH 3.8 (yellow) - 5.4 (blue). UV-Visible (lambdamax): 423 nm, 444 nm, 617 nm. Bromocresol green is also an inhibitor of prostaglandin uptake that blocks the immediate rise in intracellular immunoreactive PGE2 following treatment with 16,16-dimethyl-PGE2. Bromocresol green prevents the stimulatory effect of 16,16-dimethyl-PGE on cell proliferation, adhesion, migration and invasion and on HIF-1alpha expression and activity. Additional applications include use in sol-gel matrices and the detection of ammonia.

MFCD00005874

C21H14Br4O5S

698.01

Vial

Bromocresol green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualization of the compounds with functional groups whose pKa is below 5.0. One major use is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue). Visual transition interval: pH 3.8 (yellow) - 5.4 (blue). UV-Visible (lambdamax): 423 nm, 444 nm, 617 nm. Bromocresol green is also an inhibitor of prostaglandin uptake that blocks the immediate rise in intracellular immunoreactive PGE2 following treatment with 16,16-dimethyl-PGE2. Bromocresol green prevents the stimulatory effect of 16,16-dimethyl-PGE on cell proliferation, adhesion, migration and invasion and on HIF-1alpha expression and activity. Additional applications include use in sol-gel matrices and the detection of ammonia.

>95% (Dye content)

O=S1(C2=CC=CC=C2C(C3=CC(Br)=C(O)C(Br)=C3C)(C4=C(C)C(Br)=C(O)C(Br)=C4)O1)=O

Soluble in ethanol (30mg/ml).

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at RT.