NSJ Bioreagents

MART-1 / Melan-A Antibody

Product Code:
 
NSJ-V7772
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DT101 + BC199
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the MART-1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human melanoma with MART-1 antibody cocktail (clones DT101 + BC199). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
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IHC staining of FFPE human melanoma wit
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IHC staining of FFPE human melanoma with MART-1 antibody cocktail (clones DT101 + BC199). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
IHC staining of FFPE human melanoma wit

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CodeSizePrice
NSJ-V7772-20UG20 ug£264.00
Quantity:
NSJ-V7772-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This antibody cocktail recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the MART-1 antibody cocktail.
Limitation:
This MART-1 antibody cocktail is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q16655