NSJ Bioreagents

Folate Hydrolase 1 Antibody / FOLH1 / PSMA

Product Code:
 
NSJ-V7505
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
FOLH1/2363
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the FOLH1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 6
IHC testing of FFPE human prostate cancer with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 6
IHC testing of FFPE human prostate carcinoma with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 6
SDS-PAGE analysis of purified, BSA-free FOLH1 antibody as confirmation of integrity and purity.
4 / 6
IHC testing of FFPE rat kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
5 / 6
IHC testing of FFPE dog kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
6 / 6
IHC testing of FFPE cow brain tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate cancer with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC testing of FFPE human prostate carcinoma with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free FOLH1 antibody as confirmation of integrity and purity.
IHC testing of FFPE rat kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC testing of FFPE dog kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC testing of FFPE cow brain tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V7505-20UG20 ug£264.00
Quantity:
NSJ-V7505-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the FOLH1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 232-433 from the human protein was used as the immunogen for this FOLH1 antibody.
Limitation:
This FOLH1 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q04609