HIF1A Antibody / HIF-1 alpha
Antibody Isotype:
Mouse IgG2b, kappa
Antibody Clonality:
Monoclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
Storage:
Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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This product comes from:
United States.
Typical lead time:
10-14 working days.
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Further Information
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HIF1 alpha antibody to be titered up or down for optimal performance.
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Purified
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
3091 (Human)
Recombinant human HIF1 alpha was used as the immunogen for this antibody.
This HIF1 alpha antibody is available for research use only.
Nuclear, possible cytoplasmic
Protein G purified antibody
Human
