NSJ Bioreagents

12 Lipoxygenase Antibody / ALOX12

Product Code:
 
NSJ-F54466
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the ALOX12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human skin tissue with ALOX12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of 1) human HeLa and 2) rat spleen lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
3 / 5
Western blot testing of mouse lung lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
4 / 5
Immunofluorescent staining of human A549 cells with ALOX12 antibody (green) and anti-Actin (red).
5 / 5
Flow cytometry testing of human K562 cells with ALOX12 antibody; Blue=isotype control, Green= ALOX12 antibody.
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IHC testing of FFPE human skin tissue with ALOX12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HeLa and 2) rat spleen lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
Western blot testing of mouse lung lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
Immunofluorescent staining of human A549 cells with ALOX12 antibody (green) and anti-Actin (red).
Flow cytometry testing of human K562 cells with ALOX12 antibody; Blue=isotype control, Green= ALOX12 antibody.

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CodeSizePrice
NSJ-F54466-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54466-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ALOX12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Oxygenase and 14,15-leukotriene A4 synthase activity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 618-650 from the human protein was used as the immunogen for the ALOX12 antibody.
Limitation:
This ALOX12 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P18054