NSJ Bioreagents

SLC16A3 Antibody / MCT4

Product Code:
 
NSJ-F54278
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MCT4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human skeletal muscle tissue with MCT4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human colon carcinoma tissue with MCT4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human 1) NCI-H460, 2) HeLa, 3) HCT-116 and 4) mouse Neuro-2a cell lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.
4 / 5
Western blot testing of 1) human skeletal muscle and 2) mouse C2C12 lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.
5 / 5
Western blot testing of 1) human skeletal muscle and 2) rat skeletal muscle lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.
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IHC testing of FFPE human skeletal muscle tissue with MCT4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon carcinoma tissue with MCT4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) NCI-H460, 2) HeLa, 3) HCT-116 and 4) mouse Neuro-2a cell lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of 1) human skeletal muscle and 2) mouse C2C12 lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of 1) human skeletal muscle and 2) rat skeletal muscle lysate with MCT4 antibody. Predicted molecular weight ~50 kDa.

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CodeSizePrice
NSJ-F54278-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54278-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the MCT4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 433-462 from the human protein were used as the immunogen for the MCT4 antibody.
Limitation:
This MCT4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O15427