NSJ Bioreagents

RTCA Antibody / RTCD1

Product Code:
 
NSJ-RQ6396
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RTCA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human PC-3 cells with RTCA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) U-87 MG, 2) ThP-1 and 3) A431 cell lysate with RTCA antibody. Predicted molecular weight ~39 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with RTCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCA antibody.
❮❯

Immunofluorescent staining of FFPE human PC-3 cells with RTCA antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) U-87 MG, 2) ThP-1 and 3) A431 cell lysate with RTCA antibody. Predicted molecular weight ~39 kDa.
Flow cytometry testing of human Caco-2 cells with RTCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTCA antibody.

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CodeSizePrice
NSJ-RQ6396-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RTCA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The RTCA gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q18-L366) was used as the immunogen for the RTCA antibody.
Limitation:
This RTCA antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O00442