NSJ Bioreagents

IL-2 Receptor Antibody beta subunit / IL2RB

Product Code:
 
NSJ-RQ6188
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the IL2RB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human A549 cells with IL2RB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC staining of FFPE human appendicitis tissue with IL2RB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) HeLa, 2) HEK293, 3) K562, 4) ThP-1, 5) rat PC-12, 6) mouse intestine and 7) mouse RAW264.7 lysate with IL2RB antibody. Expected molecular weight ~61 kDa (unmodified), 70-75 kDa (glycosylated).
4 / 4
Flow cytometry testing of human U-2 OS cells with IL2RB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL2RB antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with IL2RB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human appendicitis tissue with IL2RB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HEK293, 3) K562, 4) ThP-1, 5) rat PC-12, 6) mouse intestine and 7) mouse RAW264.7 lysate with IL2RB antibody. Expected molecular weight ~61 kDa (unmodified), 70-75 kDa (glycosylated).
Flow cytometry testing of human U-2 OS cells with IL2RB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL2RB antibody.

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CodeSizePrice
NSJ-RQ6188-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IL2RB antibody should be determined by the researcher.
Description:
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. [UniProt]
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids R268-V551) was used as the immunogen for the IL2RB antibody.
Limitation:
This IL2RB antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14784