NSJ Bioreagents

LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

Product Code:
 
NSJ-V9278
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
LEF1/341R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human lymph node with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human tonsil tissue with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center cells is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.Inset: PBS instead of primary. Secondary antibody negative control.
3 / 3
SDS-PAGE analysis of purified, BSA-free LEF1 antibody (clone LEF1/341R) as confirmation of integrity and purity.
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IHC staining of FFPE human lymph node with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with LEF1 antibody (clone LEF1/341R) at 2ug/ml in PBS for 30min RT. Strong nuclear staining of non-germinal center cells is observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.Inset: PBS instead of primary. Secondary antibody negative control.
SDS-PAGE analysis of purified, BSA-free LEF1 antibody (clone LEF1/341R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9278-20UG20ug£264.00
Quantity:
NSJ-V9278-100UG100ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the LEF1 antibody should be determined by the researcher.
Description:
Lymphoid Enhancing Factor 1 (LEF1), also called T cell-specific transcription factor 1-alpha (TCF1 alpha) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 100-200 was used as the immunogen for the LEF1 antibody.
Limitation:
This LEF1 antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9UJU2