Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Show All
Further Information
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3.
Western Blot
To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.
batch dependent
Unconjugated
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing immobilized Murine IL-3 matrix.
16187
interleukin 3
Il3
Mus musculus
Lyophilized
P01586
P01586.1
IL-3-specific antibody was purified by affinity chromatography employing immobilized Murine IL-3 matrix
Infectious Disease,Cancer,Other,Immunology,Cell Cycle,Stem Cell
P01586
Centrifuge vial prior to opening.