ProSci

IL-7 Antibody

Product Code:
 
PSI-38-350
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Rat IL-7 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Rat IL-7 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.
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To detect Rat IL-7 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Rat IL-7 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.

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CodeSizePrice
PSI-38-350-0.05mg0.05mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
Lymphopoietin 1(LP-1), pre-B cell factor
Application Note:
Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IL-7 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.
Western Blot
To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-7 is a hematopoietic growth factor that primarily affects early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors, such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant Rat IL-7 is a 15.0 kDa protein containing 130 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Rat IL-7. Anti-Rat IL-7 specific antibody was purified by affinity chromatography employing an immobilized Rat IL-7 matrix.
NCBI Gene ID #:
25647
NCBI Official Name:
interleukin 7
NCBI Official Symbol:
Il7
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P56478
Protein GI Number:
P56478.1
Purification:
IL-7 specific antibody was purified by affinity chromatography employing an immobilized Rat IL-7 matrix
Research Area:
Cancer,Immunology,Stem Cell
Swissprot #:
P56478
User NOte:
Centrifuge vial prior to opening.