KRIBIOLISA™ Ovalbumin ELISA

0.5 ng/ml - 100 ng/ml

Colorimetric, 450nm

Immunoassay for the quantification of Ovalbumin Egg allergies occur in a small size of the population. As some of the vaccines like influenza and yellow fever vaccines are made in eggs, egg proteins (primarily Ovalbumin) are present in the final product. Residual quantities of egg proteins found in the vaccines may induce severe and rarely fatal hypersensitivity reactions in children with egg allergies. The KRIBIOLISA Ovalbumin ELISA enables estimation of Ovalbumin found as impurities in vaccines and cell culture supernatants. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA method. Samples containing Ovalbumin are reacted with microtiter wells pre-coated with affinity purified capture Ovalbumin antibody followed by washing and then detected using detection antibody and HRP Conjugated antibody. This immunological reaction results in formation of an immuno complex of solid phase. The wells are washed to remove any unbound reactants. The substrate 3, 3?, 5, 5? Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Ovalbumin present.

Cell Culture Supernatant and other Biological Preparations

Please refer to the current IFU for the updated calibrator range and sensitivity.