KRIBIOLISA™ HEK HCP ELISA

0 ng/ml - 200 ng/ml

Colorimetric, 450nm

Immunoassay for the measurement of HEK Host Cell Proteins. The KRIBIOLISA? ELISA kits are used for assessing the specific biomarker in samples analytes. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA procedure. Samples containing HEK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-HEK antibody in microtiter strips coated with an affinity purified capture anti-HEK antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HEK HCPs present.

Cell Culture Supernatant and other Biological Preparations

Please refer to the current IFU for the updated calibrator range and sensitivity.