Protein A Sepharose Purification Kit

Affinity purified Protein A antibodies, developed in mice, were conjugated to NHS-sepharose. Protein A is a 42 kDa surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG, and others and is, therefore, suitable for purification and detection of immunoglobulins. Therefore, Croyez Protein A sepharose is an efficient technique for IgG purification. Recombinant protein A is immobilized at over 5 mg antibody per mL 50% slurry, and this kit allows a rapid and efficient affinity purification of active IgG proteins. The affinity resin allows an efficient binding of IgG proteins without the need for preliminary steps and calibrations. The affinity-bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.