HA tagged Protein PURIFICATION KIT

The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require highly purified, active proteins for studies involving signaling pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and much more. Thus, choosing a method of purification is an important aspect in maintaining protein structure and function. Recombinant tagged protein purification methods and kits are now widely recognized. The HA epitope tag, YPYDVPDYA, is derived from Human influenza hemaglutinin (HA) 98-106aa. This HA tag is also commonly used in such as mammalian cell expression vectors. MBLI?s HA tagged Protein PURIFICATION kit is designed for the isolation of HA tagged protein from cell culture supernatants containing serum and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBLI has developed the Anti-HA tag Beads to purify HA tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of HA tagged proteins from the Beads is achieved by the addition of the HA peptide. As the HA peptide competes with HA tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.

Anti-HA tag Beads, Elution Peptide, Spin Columns Sets, Wash Concentrate

1 year

HA