MBL

pFucci-S/G2/M Green-Hyg (Expression vector)

Product Code:
 
MBL-AM-V9010M
Supplier:
 
MBL
Regulatory Status:
 
RUO
Shipping:
 
4°C
Storage:
 
-20°C
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MBL-AM-V9010M20 ug£845.00
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Further Information

Background:
AM-V9010 pFucci-S/G2/M Green-Hyg is a mammalian expression vector encoding CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). ?Fucci? stands for Fluorescent Ubiquitination-based Cell Cycle Indicator. Geminin is an inhibitor of the DNA replication licensing factor. It accumulates during the S, G2, and M phases, but is degraded during G1 phase by ubiquitin-mediated proteolysis. A part of hGeminin (1-110) is also degradable in a cell cycle dependent manner. CoralHue® hmAG1 sequence is codon-optimized for higher expression in mammalian cells. CoralHue® monomeric AG1 (mAG1) has been generated from tetrameric CoralHue® Azami-Green (AG).
Formulation:
Dry form. Reconstitute with distilled water or TE before use.
Gene Accession Number:
Accession Numbers: AB370333
Purity:
A260/A280 > 1.5
Source:
The CoralHue® AG gene was cloned from a stony coral (Galaxea fascicularis).
Target:
pFucci-S/G2/M

Documents

References

Sakaue-Sawano, A., et al., Cell. 132, 487-498 (2008) Nakayama, K. I., et al., Nat. Rev. Cancer. 6, 369-381 (2006) Blow, J. J., and Dutta, A., Nat. Rev. Mol. Cell Biol. 6, 476-486 (2005) Nishitani, H., et al., J. Biol. Chem. 279, 30807-30816 (2004) Karasawa, S., et al., J. Biol. Chem. 278, 34167-71 (2003) Nishitani, H., et al., Nature. 404, 625-628 (2000)