MBL

Anti-Phospho-Syntide-2 mAb

Product Code:
 
MBL-CY-M1023
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MS-6E6
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 1

No additional charges, what you see is what you pay! *

CodeSizePrice
MBL-CY-M1023100 ug£301.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Show All

Further Information

Applications:
ELISA - 1 ug/mL
Background:
Syntide-2, a peptide based on phosphorylation site two of glycogen synthase, is an exogenous substrate designed for CaM-Kinase (calcium/calmodulin dependent kinases) in the serine-threonine kinase family. CaM-kinase II is a multifunctional calcium/calmodulin dependent protein kinase involved in neuronal functions. This anti-Phospho-Syntide-2 monoclonal antibody has been validated with CaM-kinase II, however it has the potential for use in evaluating other serine/threonine kinases such as CaM-Kinase IIa, PKCu, Akt1, Akt2, Akt3, and PKA. The relative Vmax/Km ratios of the known Ca2+-dependent protein kinases for syntide-2 were determined to be as follows: protein kinase II, 100; protein kinase C, 22; phosphorylase kinase, 2; myosin light chain kinase, 0.005.
Concentration:
1.0 mg/mL
Formulation:
Supplied in 20mM phosphatase buffer (pH 7.5), 300mM NaCl, 50% glycerol.
Immunogen Translated:
a synthetic phosphopeptide Syntide-2 (PLARTL(pS)VAGLPGKK; synthetic substrate for CaM-Kinase II)
Reactivity:
Phospho-Syntide-2 Antibody detects phosphorylated Syntide-2 only when phosphorylated at the serine residue in vitro. The antibody does not recognize the unphosphorylated substrate. The antibody detects the phosphorylated Syntide-2 by ELISA.
Shelf Life:
1 year
Source:
This monoclonal antibody (clone MS-6E6) was produced by immunizing mice with a synthetic phosphopeptide Syntide-2, PLARTL(pS)VAGLPGKK, which is synthetic substrate for CaM-Kinase II (Calmodulin dependent protein kinase II). The IgG fraction was purified by protein A-Sepharose chromatography.
Target:
Syntide-2

Documents

References

1) Hashimoto Y, Soderling TR. Calcium calmodulindependent protein kinase II and calcium phospholipid-dependent protein kinase activities in rat tissues assayed with a synthetic peptide. Arch Biochem Biophys. 252:418-25, (1987) 2) CM Schworer, RJ Colbran, and TR Soderling Reversible generation of a Ca2+-independent form of Ca2+(calmodulin)- dependent protein kinase II by an autophosphorylation mechanism. J. Biol. Chem. 261:8581 ? 8584, (1986)