Elabscience

Vitamin C (VC) Colorimetric Assay Kit

Product Code:
 
E-BC-K034-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Quantification
Shipping:
 
Ice packs
Storage:
 
2-8°C
1 / 2
2 / 2

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  • Further Information
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Further Information

Assay Time:
50min
Detection Instrument:
Microplate reader
Detection Range:
0.31-20 ug/mL
Detection Type:
Colorimetric method
Inter cv:
6.1
Intra cv:
2.2
Result Type:
Quantitative
Sensitivity:
0.31 ug/mL
Target Synonym:
VC;Ascorbic acid
Test Principle:
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Tested Sample Types:
Serum,plasma,tissue

Documents

References

  • https://www.mdpi.com/article/10.3390/nu15040832
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403741/
  • https://www.nature.com/articles/s41598-019-52646-z
  • https://www.sciencedirect.com/science/article/pii/S0024320520316295
  • https://www.tandfonline.com/doi/abs/10.2147/IJGM.S402206
  • https://www.sciencedirect.com/science/article/pii/S2405457721001091