Elabscience

QuicKey Pro Human IL-8(Interleukin 8) ELISA Kit

Product Code:
 
E-OSEL-H0014
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice packs
Storage:
 
An unopened kit can be stored at 2-8°C for 6 months. After test the unused wells and reagents should be stored according to the storage table in the manual.
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Standard curve
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Standard curve

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E-OSEL-H0014-24T24T£188.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-OSEL-H0014-48T48T£285.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-OSEL-H0014-96T96T£355.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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This product comes from: China.
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  • Further Information
  • Documents
  • References
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Further Information

Abbreviation:
IL-8
Assay Time:
1.5h
Cross reactivity:
This kit recognizes Human IL-8 in samples.No significant cross-reactivity or interference between Human IL-8 and analogues was observed
Detection Range:
15.63-1000 pg/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
9.38 pg/mL
Target Species:
Human
Target Synonym:
IL8/CXCL8
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-8 are added to the micro ELISA plate wells. Human IL-8 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-8.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value is proportional to the concentration of Human IL-8. The concentration of Human IL-8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma
Type:
Sandwich
UNIProt ID:
P10145
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma.

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S009286742031000X
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/
  • https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6
  • https://www.sciencedirect.com/science/article/pii/S0304383522000040
  • https://www.nature.com/articles/s41419-022-04734-8
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351451/
  • https://www.nature.com/articles/s41416-022-01744-3
  • https://www.mdpi.com/2072-6694/15/5/1510
  • https://www.frontiersin.org/articles/10.3389/fcell.2020.574940/full
  • https://www.sciencedirect.com/science/article/pii/S0944711322004184
  • https://www.mdpi.com/1964258
  • https://www.mdpi.com/1422-0067/23/22/13755