Elabscience

Flag-Tag Monoclonal Antibody

Product Code:
 
E-AB-20006
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Clone:4A2
Regulatory Status:
 
RUO
Applications:
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Monoclonal Antibody at dilution of 1:10000.
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Immunofluorescence analysis of 293 cells transfected with a Flag tag protein tissue using Flag-Tag Monoclonal Antibody at dilution of 1:2000.
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Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Monoclonal Antibody at dilution of 1:10000.
Immunofluorescence analysis of 293 cells transfected with a Flag tag protein tissue using Flag-Tag Monoclonal Antibody at dilution of 1:2000.

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E-AB-20006-30uL30uL£125.00
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E-AB-20006-60uL60uL£146.00
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E-AB-20006-120uL120uL£196.00
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E-AB-20006-200uL200uL£216.00
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This product comes from: China.
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  • Further Information
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Further Information

Abbreviation:
Flag-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. 
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Immunogen:
Synthetic Peptide
Purification method:
Protein A purification
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag

Documents

References

  • https://www.frontiersin.org/articles/10.3389/fpls.2021.713544/full