Elabscience

CASP8 Monoclonal Antibody

Product Code:
 
E-AB-22107
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Clone:2E3
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western Blot analysis of 1) Hela, 2) Mouse brain, 3) Rat brain with CASP8 Monoclonal Antibody
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Immunohistochemistry of paraffin-embedded Mouse spleen tissue with CASP8 Monoclonal Antibody
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Western Blot analysis of 1) Hela, 2) Mouse brain, 3) Rat brain with CASP8 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded Mouse spleen tissue with CASP8 Monoclonal Antibody

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E-AB-22107-20uL20uL£125.00
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E-AB-22107-60uL60uL£172.00
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E-AB-22107-120uL120uL£237.00
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E-AB-22107-200uL200uL£344.00
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  • Further Information
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Further Information

Abbreviation:
Caspase-8
Background:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Immunogen:
Recombinant Protein
ObservedMW:
43,57kDa
Purification method:
Protein A purification
Target Synonym:
ALPS2B;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein;Apoptotic cysteine protease;Apoptotic protease Mch-5;Apoptotic protease Mch5;CAP4;CASP-8;CASP8;CASP8;Caspase 8;Caspase 8 apoptosis related cysteine peptidase;Caspase-8 subunit p10;CED 3;FADD Like ICE;FADD-homologous ICE/CED-3-like protease;FADD-like ICE;FLICE;FLJ17672;ICE-like apoptotic protease 5;MACH alpha 1/2/3 protein;MACH;MACH beta 1/2/3/4 protein;MCH5;MGC78473;MORT1 associated ced 3 homolog;MORT1-associated CED-3 homolog;OTTHUMP00000163717;OTTHUMP00000163720;OTTHUMP00000163724;OTTHUMP00000163725;OTTHUMP00000165062;OTTHUMP00000165063;OTTHUMP00000165064;OTTHUMP00000206552;OTTHUMP00000206582
UNIProt ID:
Q14790

Documents

References

  • https://www.frontiersin.org/articles/10.3389/fonc.2021.735183/full
  • https://www.mdpi.com/1422-0067/23/18/10411
  • https://www.nature.com/articles/s41419-018-0449-5
  • https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jat.4286