Mouse β-EP(Beta-Endorphin) ELISA Kit

β-EP

2.5h

This kit recognizes Mouse β-EP in samples.No significant cross-reactivity or interference between Mouse β-EP and analogues was observed

15.63-1000 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

9.38 pg/mL

Mouse

b-EP

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. During the reaction, Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Mouse β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Competitive

P01193

This ELISA kit applies to the in vitro quantitative determination of Mouse β-EP concentrations in serum, plasma and other biological fluids.