Elabscience

NADP+/NADPH Colorimetric Assay Kit

Product Code:
 
E-BC-K803-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
-20°C
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E-BC-K803-M-96T96T£324.90£361.00
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Further Information

Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
0.02-5.0 umol/L
Detection Type:
Colorimetric method
Inter cv:
5.5
Intra cv:
2.1
Result Type:
Quantitative
Sensitivity:
0.02 umol/L
Target Synonym:
NADP;NADPH
Test Principle:
Detect total content of NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60°C water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH.
Tested Sample Types:
animal tissue, cells

Documents

References

  • https://www.mdpi.com/2076-3921/11/9/1692
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/bit.28222
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/jdi.13690
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.23740