Recombinant Mouse SRC Kinase/c-SRC Protein (His & GST Tag)(Active)

SRC Kinase;c-SRC

NP_001020566.111

1. The specific activity was determined to be > 80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate. 2. Immobilized recombinant Mouse SRC at 2 ug/ml (100 ul/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 ug/ml.

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

87.7 kDa

< 1.0 EU per ug of the protein as determined by the LAL method.

Baculovirus-Insect Cells

Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

N-His-GST

80 kDa

> 95 % as determined by reducing SDS-PAGE.

Met 1-Leu 535

AW259666;pp60c-src