Human PPY(Pancreatic polypeptide) ELISA kit

PPY

3.5h

This kit recognizes Human PPY in samples. No significant cross-reactivity or interference between Human PPY and analogues was observed.

125-8000 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

40.12 pg/mL

Human

PNPPancreatic prohormone; Pancreatic polypeptide; PP; Pancreatic icosapeptide

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human PPY. You can calculate the concentration of Human PPY in the samples by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Sandwich

P01298

This ELISA kit applies to the in vitro quantitative determination of Human PPY concentrations in serum, plasma and other biological fluids.