NSJ Bioreagents

Paxillin Antibody

Product Code:
 
NSJ-R20277
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM256
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store the recombinant Paxillin antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 3
Western blot testing of human HeLa cell lysate with recombinant Paxillin antibody at 1:1000. Predicted molecular weight: 61/65/66 kDa (alpha/beta/gamma).
2 / 3
ICC/IF staining of HeLa cells using recombinant Paxillin antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
3 / 3
IHC testing of FFPE human tonsil tissue with recombinant Paxillin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HeLa cell lysate with recombinant Paxillin antibody at 1:1000. Predicted molecular weight: 61/65/66 kDa (alpha/beta/gamma).
ICC/IF staining of HeLa cells using recombinant Paxillin antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus labeled with DAPI (blue).
IHC testing of FFPE human tonsil tissue with recombinant Paxillin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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NSJ-R20277-0.1ML100ul£524.00
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Further Information

Application Details:
Immunocytochemistry: 1:1000-1:2000,Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant Paxillin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This recombinant Paxillin antibody reacts to human Paxillin. It may also react to mouse or rat Paxillin, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the N-terminus of human Paxillin was used as the immunogen for this recombinant Paxillin antibody.
Limitation:
This recombinant Paxillin antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P49023

Documents