NSJ Bioreagents

CD146 Antibody

Product Code:
 
NSJ-R20269
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RM249
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD146 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
1 / 2
Western blot testing of human A375 cell lysate with recombinant CD146 antibody at 1:1000. Observed molecular weight 70-120 kDa depending on glycosylation level.~
2 / 2
IHC testing of FFPE human breast cancer tissue with recombinant CD146 ant
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Western blot testing of human A375 cell lysate with recombinant CD146 antibody at 1:1000. Observed molecular weight 70-120 kDa depending on glycosylation level.~
IHC testing of FFPE human breast cancer tissue with recombinant CD146 ant

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NSJ-R20269-0.1ML100ul£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:200-1:500 (1),Western blot: 1:1000-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant CD146 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Description:
This recombinant CD146 antibody reacts to human CD146 (Cell surface glycoprotein MUC18). It may also react to the mouse and rat protein, as predicted by immunogen homology.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to the residues near C-terminus of human CD146 was used as the immunogen for this recombinant CD146 antibody.
Limitation:
This recombinant CD146 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P43121

Documents