NSJ Bioreagents

Ku70 + Ku80 Antibody

Product Code:
 
NSJ-V2128CF488
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KU729
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the Ku70 + Ku80 antibody at 2-8oC protected from light.
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FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= Ku70 + Ku80 antibody.
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FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= Ku70 + Ku80 antibody.

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NSJ-V2128CF488-100T100 Tests£534.00
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Further Information

Application Details:
Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
Application Note:
Optimal dilution of the Ku70 + Ku80 antibody should be determined by the researcher.
Description:
Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Format:
CF488 Conjugate
Formulation:
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Limitation:
This Ku70 + Ku80 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography

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