NSJ Bioreagents

c-Kit Antibody / CD117

Product Code:
 
NSJ-V2167SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C117/370
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD117 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Formalin-paraffin human gastrointestinal stromal tumor (GIST) stained with CD117 antibody (clone C117/370).
2 / 3
SDS-PAGE analysis of purified, BSA-free CD117 antibody (clone C117/370) as confirmation of integrity and purity.
3 / 3
Formalin-paraffin human gastrointestinal stromal tumor (GIST) stained with CD117 antibody (clone C117/370).
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Formalin-paraffin human gastrointestinal stromal tumor (GIST) stained with CD117 antibody (clone C117/370).
SDS-PAGE analysis of purified, BSA-free CD117 antibody (clone C117/370) as confirmation of integrity and purity.
Formalin-paraffin human gastrointestinal stromal tumor (GIST) stained with CD117 antibody (clone C117/370).

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NSJ-V2167SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Description:
This antibody recognizes a protein of 145kDa, identified as CD117, or p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, CD117 antibody has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi�s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. CD117 antibody is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
3815
Immunogen:
Recombinant human protein was used as the immunogen for this CD117 antibody.
Limitation:
This CD117 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography

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