ELK Biotechnology

Rabbit NFKB-p65 (NuclearFactor Kappa B p65) ELISA Kit

Product Code:
 
ELK0584
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Rabbit
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK0584-48T48T£298.00
Quantity:
ELK0584-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
46.88-3000 pg/mL
Sample Type:
tissue homogenates, cell lysates and other biological fluids
Sensitivity:
18.1 pg/mL
Standard:
3000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rabbit NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.