NSJ Bioreagents

DNMT3B Antibody

Product Code:
 
NSJ-RQ7164
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DNMT3B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Flow cytometry testing of human Caco-2 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
2 / 4
Flow cytometry testing of mouse ANA-1 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
3 / 4
Flow cytometry testing of rat C6 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
4 / 4
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human K562, 4) monkey COS-7, 5) rat liver, 6) rat heart, 7) mouse liver and 8) mouse heart tissue lysate with DNMT3B antibody. Predicted molecular weight: 95 kDa.
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Flow cytometry testing of human Caco-2 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
Flow cytometry testing of mouse ANA-1 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
Flow cytometry testing of rat C6 cells with DNMT3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNMT3B antibody.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human K562, 4) monkey COS-7, 5) rat liver, 6) rat heart, 7) mouse liver and 8) mouse heart tissue lysate with DNMT3B antibody. Predicted molecular weight: 95 kDa.

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NSJ-RQ7164-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DNMT3B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DNA (cytosine-5-)-methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human DNMT3B protein (amino acids E271-E853) was used as the immunogen for the DNMT3B antibody.
Limitation:
This DNMT3B antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P50570

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