NSJ Bioreagents

Biotinylated HA-Tag Antibody

Product Code:
 
NSJ-R20304BTN
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM305
Regulatory Status:
 
RUO
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Store the recombinant biotinylated HA-Tag antibody at -20°C.
1 / 2
Western blot testing of lysate from human 293T cells transfected with HA-tagged KRas protein with biotinylated HA-Tag antibody at 0.1ug/ml.~
2 / 2
IHC staining of formalin fixed and paraffin embedded human LNCaP cells overexpressing HA-tag
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Western blot testing of lysate from human 293T cells transfected with HA-tagged KRas protein with biotinylated HA-Tag antibody at 0.1ug/ml.~
IHC staining of formalin fixed and paraffin embedded human LNCaP cells overexpressing HA-tag

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CodeSizePrice
NSJ-R20304BTN-50UG50 ug£524.00
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Further Information

Application Details:
Western blot: 0.1ug/ml-1ug/ml,Immunoprecipitation: 0.5ug/ml-2ug/ml,Immunocytochemistry 0.1ug/ml-1ug/ml,Immunohistochemistry (FFPE): 0.01ug/ml-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant biotinylated HA-Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.
Format:
Biotin Conjugate
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106) was used as the immunogen for the biotinylated HA-Tag antibody.
Limitation:
This recombinant biotinylated HA-Tag antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant

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