anti-NS5B (HCV) mAb (5B-3B1)

Product Code:

AG-20B-0002

Product Group:

Primary Antibodies

Supplier:

AdipoGen Life Sciences

Host Type:

Mouse

Antibody Isotype:

IgG2b

Antibody Clonality:

Monoclonal

Antibody Clone:

5B-3B1

Regulatory Status:

RUO

Target Species:

Hepatitis C virus (HCV)

Application:

Western Blot (WB)

Shipping:

Blue Ice

Storage:

-20°C

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Code	Size	Price

AG-20B-0002-C100

100 ug

£300.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Alternate Names/Synonyms:

Nonstructural Protein 5B

Concentration:

1mg/ml

EClass:

32160000

Form (Short):

liquid

Formulation:

Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Handling Advice:

After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.

Immunogen:

Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).

Long Description:

Monoclonal Antibody. Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a. Isotype: Mouse IgG2b. Clone: 5B-3B1. Applications: WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.

NCBI, Uniprot Number:

N/A

Package Type:

Plastic Vial

Product Description:

The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.

Purity:

>95% (SDS-PAGE)

Source / Host:

Purified from concentrated hybridoma tissue culture supernatant.

Specificity:

Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a.

Transportation:

Non-hazardous

UNSPSC Category:

Primary Antibodies

UNSPSC Number:

12352203

Use & Stability:

Stable for at least 1 year after receipt when stored at -20°C.

Documents

References

Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002) | Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells: R.W. Hardy, et al.; J. Virol. 77, 2029 (2003) | Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs: G. Randall, et al.; PNAS 100, 235 (2003) | Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005) | Quantitative analysis of the hepatitis C virus replication complex: D. Quinkert, et al.; J. Virol. 79, 13594 (2005) | Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3: Y.V. Svitkin, et al.; J. Virol. 79, 6868 (2005)