AdipoGen Life Sciences

anti-NS5B (HCV) mAb (blocking) (5B-12B7)

Product Code:
 
AG-20B-0003
Product Group:
 
Primary Antibodies
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5B-12B7
Regulatory Status:
 
RUO
Target Species:
 
Hepatitis C virus (HCV)
Applications:
  • Functional Study
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
Shipping:
 
Blue Ice
Storage:
 
-20°C
 

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AG-20B-0003-C100100 ug£300.00
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Further Information

Alternate Names/Synonyms:
Nonstructural Protein 5B
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.
Long Description:
Monoclonal Antibody. Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392). Does not react with genotype 2. Isotype: Mouse IgG2a. Clone: 5B-12B7. Applications: FUNC (Blocking), ICC, IP. Liquid. In PBS containing 0.02% sodium azide. The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
NCBI, Uniprot Number:
N/A
Package Type:
Plastic Vial
Product Description:
The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392). Does not react with genotype 2.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase: J. Schmidt-Mende, et al.; J. Biol. Chem. 276, 44052 (2001) | Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase: D. Moradpour, et al.; J. Biol. Chem. 277, 593 (2002) | Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003) | Interference of hepatitis C virus RNA replication by short interfering RNAs: Kapadia, et al.; PNAS 100, 2014 (2003)