AdipoGen Life Sciences

anti-Asc pAb (AL177)

Product Code:
 
AG-25B-0006
Product Group:
 
Primary Antibodies
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Functional Study
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
BLUE ICE
Storage:
 
Short Term Storage: +4?C. Long Term Storage: -20?C
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Western blot analysis of human and mouse cell lines using anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006). Total protein extracts from various human (293-T, Jurkat, Raj, Ramos, BJAB, THP-1, U937, K562, Raw, HeLa) and mouse (EL-4, A20) cell lines were
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Western blot analysis of human and mouse cell lines using anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006). Total protein extracts from various human (293-T, Jurkat, Raj, Ramos, BJAB, THP-1, U937, K562, Raw, HeLa) and mouse (EL-4, A20) cell lines were

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AG-25B-0006-C100100 ug£390.00
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Further Information

Alternate Names/Synonyms:
Pycard; Target of Methylation-induced Silencing 1; TMS1; Caspase Recruitment Domain-containing Protein 5; Apoptosis-associated Speck-like Protein Containing CARD; CARD5
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Synthetic peptide corresponding to aa at the N-terminal human Asc.
Long Description:
Polyclonal Antibody. Recognizes human and mouse Asc. Source: Rabbit. Applications: FUNC, ICC, IHC, IP, WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.
NCBI, Uniprot Number:
Q9ULZ3
Package Type:
Plastic Vial
Product Description:
Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.
Source / Host:
Rabbit
Specificity:
Recognizes human and mouse Asc.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004) | P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008) | Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183, 4003 (2009) (IF) | Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012) | NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 674 (2013) (IF) | Measuring inflammasome activation in response to bacterial infection: P. Broz & D.M. Monack; Methods Mol. Biol. 1040, 65 (2013) | Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation: J.K. Dowling, et al.; J. Biol. Chem. 289, 6429 (2014) | Localization and functionality of the inflammasome in neutrophils: M. Bakele, et al.; J. Biol. Chem. 289, 5320 (2014) | The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) (IF) | The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014) | Aging-associated TNF production primes inflammasome activation and Nlrp3-related metabolic disturbances: F. Bauernfeind, et al.; J. Immunol. 197, 2900 (2016) | Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence: M. Beilharz, et al.; Methods Mol. Biol. 1417, 145 (2016) | Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016) | The inflammasome pathway in stable COPD and acute exacerbations: R. Faner, et al.; ERJ Open Res. 2, 00002-2016 (2016) (IF) | A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly: F.I. Schmidt, et al.; J. Exp. Med. 213, 771 (2016) (IF) | Microglia-derived ASC specks cross-seed amyloid-beta in Alzheimer's disease: C. Venegas, et al.; Nature 552, 355 (2017) (IF) | The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer: E. Brunetto, et al.; J. Immunother. Cancer 7, 45 (2019) (IF) | NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) (IF) | Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, 482 (2021) | DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response: S. Kesavardhana, et al.; J. Biol. Chem. 296, 100579 (2021)