Further Information
CCR3 Antibody: CKR3, CD193, CMKBR3, CC-CKR-3, C-C chemokine receptor type 3, Eosinophil eotaxin receptor, C-C CKR-3
CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
CCR3 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells. CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS. High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems. The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.
- Feng Y, Broder CC, Kennedy PE, et al. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996; 272:872-7.
- Deng H, Liu R, Ellmeier W, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996; 381:661-6.
- Choe H, Farzan M, Sun Y, et al. The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 1996; 85:1135-48.
- He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature 1997; 385:645-9.
CCR3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CCR3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human CCR3.
The immunogen is located within the last 50 amino acids of CCR3.
1232
chemokine (C-C motif) receptor 3
CCR3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39, 41 kDa
Observed: 52 kDa
NP_847899
30581170
CCR3 Antibody is affinity chromatography purified via peptide column.
Chemokines & Cytokines,Neuroscience,Cancer,Infectious Disease
At least three isoforms of CCR3 are known to exist; this antibody will detect all three isoforms.
P51677
Optimal dilutions for each application to be determined by the researcher.