ProSci

p90RSK Antibody

Product Code:
 
PSI-79-148
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using p90RSK (Phospho-Thr348).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK (Phospho-Thr348).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using p90RSK (Phospho-Thr348).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK (Phospho-Thr348).

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PSI-79-148-0.1mg0.1mg£488.00
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Further Information

Additional Names:
RSK, HU-1, RSK1, MAPKAPK1A, MEK3, MKK3, PRKMK3, SKK2, KS6A1, MAPKAP-K1a, RPS6KA1, S6K-alpha 1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
Background References:
  • Silverman E, et al. Mol Cell Biol. 2004 Dec; 24 (24): 10573-10583. Andrew D, et al. Biochem J. 2006 February 1; 393 (Pt 3): 715?724. Itoh S, et al. J Biol Chem 2005 Jun 24; 280 (25): 24135-24142
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p90RSK (Phospho-Thr348) antibody was raised against a peptide sequence around phosphorylation site of threonine 348 (S-R-T (p) -P-R) derived from Human p90RSK.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6195
NCBI Official Name:
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol:
RPS6KA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
NP_001006666.1
Protein GI Number:
55743134
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348.
Swissprot #:
Q15418