Further Information
DDAH2, dimethylarginine dimethylaminohydrolase 2, DDAH, G6a, NG30, DDAHII, dimethylarginine dimethylaminohydrolase II, NG-dimethylarginine dimethylamino hydrolase homolog, OTTHUMP00000062666, DADB-110M10.5, OTTHUMP00000029307, OTTHUMP00000174406, alternative name: NG30, G6a, dimethylarginine dimethylaminohydrolase II
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3?g/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.Flow Cytometry:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 1999 Oct 1;343 Pt 1:209-14
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: SSLCLVLSTRPHS
23564
dimethylarginine dimethylaminohydrolase 2
DDAH2
Homo sapiens
Liquid
NP_039268.1
7524354
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer ,Infectious Disease
.
O95865