ProSci

PRMT3 Antibody

Product Code:
 
PSI-43-047
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
43-047 (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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43-047 (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-43-047-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
PRMT3, protein arginine methyltransferase 3, HRMT1L3, HMT1 hnRNP methyltransferase-like 3, OTTHUMP00000231732, OTTHUMP00000231735, heterogeneous nuclear ribonucleoprotein methyltransferase-like 3, heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, protein arginine N-methyltransferase 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in lysates of cell line MCF7 (calculated MW of 59.9kDa according to NP_005779.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Swiercz et al, J Biol Chem. 2007 Jun 8;282(23):16917-23. PMID: 17439947). Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: DPKTLISEPCGIKH
NCBI Gene ID #:
10196
NCBI Official Name:
protein arginine methyltransferase 3
NCBI Official Symbol:
PRMT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70 kDa
Protein Accession #:
NP_005779.1, NP_001138638.1, NP_001138639.1
Protein GI Number:
44771198
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).
Swissprot #:
O60678