Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IgG monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, IgG Fc receptor II-a, FcGR, CD32A, FCGR2
Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
- Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
- Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).
Purified antibody in PBS containing 0.03% sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Chrompure Human IgG, Fc Fragment.
2212
Fc fragment of IgG, low affinity IIa, receptor (CD32)
FCGR2A
Homo sapiens
P12318
160332371
Antibody is purified by protein A affinity chromatography.
Immunology
P12318
Optimal dilutions for each application to be determined by the researcher.